Salk J, Stoeckel P, van Wezel A L, Lapinleimu K, van Steenis G
Ann Clin Res. 1982 Dec;14(5-6):204-12.
The immunologic response to inactivated poliovirus vaccine following one and two doses has been studied in infants in developing and developed countries using vaccine prepared at the Rijks Instituut voor de Volksgezondheit, The Netherlands. Virus was grown in microcarrier cultures of monkey kidney cells, purified, concentrated, and inactivated with formalin. The vaccines used contained different quantities of D-antigen units for each of the three types. The data reveal that both antibody and immunologic memory (booster-type responsiveness) were induced in virtually all individuals following a single dose of a sufficient quantity of antigen. Immunologic memory was readily revealed by the booster-type response following a second dose given six months after the first. The degree of booster-type response to a second dose is linked primarily to the quantity of antigen used for primary immunization, and secondarily to the quantity of antigen used for the booster dose. The data base is presented for formulating the antigen content of an inactivated poliovirus vaccine that can be relied upon to be protective after the first dose when given alone or when incorporated with combinations of other antigens (diphtheria-per tussis-tetanus) that may require two or more doses.
在发展中国家和发达国家的婴儿中，使用荷兰国家公共卫生研究所制备的疫苗，对一剂和两剂灭活脊髓灰质炎疫苗后的免疫反应进行了研究。病毒在猴肾细胞的微载体培养物中生长，经过纯化、浓缩，并用福尔马林灭活。所使用的疫苗对三种类型中的每一种都含有不同数量的D抗原单位。数据显示，在几乎所有个体中，单剂量给予足够数量的抗原后，均可诱导抗体和免疫记忆（加强型反应性）。在首剂接种六个月后给予第二剂，通过加强型反应很容易揭示免疫记忆。对第二剂的加强型反应程度主要与用于初次免疫的抗原量有关，其次与用于加强剂量的抗原量有关。本文提供了一个数据库，用于制定一种灭活脊髓灰质炎疫苗的抗原含量，这种疫苗在单独给予首剂时或与其他可能需要两剂或更多剂的抗原（白喉-百日咳-破伤风）联合使用时，都可被认为具有保护作用。
