Feit H, Domke R
Cell Motil. 1982;2(3):309-15. doi: 10.1002/cm.970020309.
The isotype pattern of tropomyosin was investigated in normal and dystrophic avian pectoral muscle using two-dimensional gel electrophoresis. Previous reports have shown that adult pectoral muscle of chickens contains only the alpha-subunit of tropomyosin and a breast-type troponin-T (TN-T), whereas pectoral fetal muscle contains both alpha-and beta-tropomyosin and leg-type TN-T. The change from the fetal to the adult forms begins shortly after hatching. It has been previously reported that avian dystrophic pectoral muscle contains both the leg- and breast-type TN-T; we show that in avian dystrophic muscle there is also persistent expression of the beta-subunit of tropomyosin.
利用二维凝胶电泳技术，对正常和营养不良的禽胸肌中原肌球蛋白的同型模式进行了研究。先前的报道表明，成年鸡的胸肌仅含有原肌球蛋白的α亚基和一种胸型肌钙蛋白-T（TN-T），而胎儿胸肌则同时含有α和β原肌球蛋白以及腿型TN-T。从胎儿型到成年型的转变在孵化后不久就开始了。此前有报道称，禽营养不良胸肌同时含有腿型和胸型TN-T；我们发现，在禽营养不良肌肉中，原肌球蛋白的β亚基也持续表达。
