Gormus B J, Vessella R L, Martin L N, Kaplan M E
Comp Immunol Microbiol Infect Dis. 1982;5(4):483-99. doi: 10.1016/0147-9571(82)90074-1.
We have studied the ability of human peripheral blood lymphocytes (HuPBL)4 to interact with IgG from several animal species. Three functions or activities that are reported to depend on an interaction between complexed IgG and HuPBL receptors (R) for the Fc piece of IgG (Fc gamma R) were compared: (1) antibody-dependent cell-mediated cytotoxicity (ADCC); (2) binding of heat-aggregated IgG (aggG); and (3) rosette formation with IgG-sensitized erythrocytes [RBC-A(gamma)]. IgG (and IgM) antibodies to chicken erythrocytes (CRBC) were purified from the sera of the following species after injection with CRBC stroma: (1) horse (Ho); (2) goat (Go); (3) rabbit (Ra); and (4) guinea pig (Gp). Good IgG-agglutinating antibody titers were obtained from each injected species. Using 51Cr-labeled CRBC targets and HuPBL effector cells, only Ra anti-CRBC IgG gave good ADCC at high dilutions. Ho and Go anti-CRBC (IgG) failed to give ADCC, and Gp anti-CRBC (IgG) gave approx. 30% of the level of kill as Ra. Ra Fab2 fragments of IgG antibody failed to produce ADCC. Treatment of HuPBL with Ra anti-lymphocyte serum (ALS) almost totally ablated ADCC, whereas HoALS failed to alter ADCC. Pretreatment of HuPBL with aggG showed that Ra or Hu aggG gave essentially equal inhibition of ADCC, Gp gave approx. 30% of the degree of inhibition as Hu and Ra, and Ho or Go aggG had essentially no effect of ADCC. These results confirmed the following order of ability of IgG to interact with HuPBL ADCC killer (K) cells: (Hu greater than or equal to)Ra greater than Gp much greater than Ho, Go. The data suggest that Gp IgG interacts with only a subpopulation (approximately 30%) of HuPBL K cells. The binding of aggG to total HuPBL failed to strictly correlate with the ADCC results or with the results of rosette formation between total HuPBL and CRBC-A(gamma). The observations suggest that there is a heterogeneity of Fc gamma R between K and non-K cell subpopulations of HuPBL both in terms of the type of complexed IgG they are able to bind, and in terms of species of origin of the IgG. The data also support contentions that Fc gamma R that bind RBC-A (gamma) complexes differ from those that bind aggG.
我们研究了人外周血淋巴细胞（HuPBL）与几种动物物种的IgG相互作用的能力。比较了据报道依赖于复合IgG与HuPBL上IgG Fc片段受体（R）（FcγR）之间相互作用的三种功能或活性：（1）抗体依赖性细胞介导的细胞毒性（ADCC）；（2）热聚集IgG（aggG）的结合；（3）与IgG致敏红细胞[RBC-A(γ)]形成玫瑰花结。向以下物种注射鸡红细胞（CRBC）基质后，从其血清中纯化了针对鸡红细胞的IgG（和IgM）抗体：（1）马（Ho）；（2）山羊（Go）；（3）兔（Ra）；（4）豚鼠（Gp）。从每个注射物种中都获得了良好的IgG凝集抗体效价。使用51Cr标记的CRBC靶细胞和HuPBL效应细胞，只有Ra抗CRBC IgG在高稀释度下能产生良好的ADCC。Ho和Go抗CRBC（IgG）未能产生ADCC，而Gp抗CRBC（IgG）产生的杀伤水平约为Ra的30%。IgG抗体的Ra Fab2片段未能产生ADCC。用Ra抗淋巴细胞血清（ALS）处理HuPBL几乎完全消除了ADCC，而HoALS未能改变ADCC。用aggG预处理HuPBL表明，Ra或Hu aggG对ADCC的抑制作用基本相同，Gp的抑制程度约为Hu和Ra的30%，而Ho或Go aggG对ADCC基本没有影响。这些结果证实了IgG与HuPBL ADCC杀伤（K）细胞相互作用能力的以下顺序：（Hu≥）Ra＞Gp＞＞Ho，Go。数据表明，Gp IgG仅与HuPBL K细胞的一个亚群（约30%）相互作用。aggG与总HuPBL的结合与ADCC结果或总HuPBL与CRBC-A(γ)之间的玫瑰花结形成结果没有严格的相关性。这些观察结果表明，HuPBL的K细胞和非K细胞亚群之间的FcγR在它们能够结合的复合IgG类型以及IgG的来源物种方面存在异质性。数据还支持这样的观点，即结合RBC-A(γ)复合物的FcγR与结合aggG的FcγR不同。
