Accelerated turnover of hepatic glucokinase in starved and streptozotocin-diabetic rat.

Rat liver glucokinase synthesis and degradation was estimated in fasted/fed and diabetic/diabetic-insulin-treated rats by the radioimmunological technique. Starvation and Streptozotocin-diabetes led to basal rates of synthesis and, consequently, to low levels in enzyme activity. In addition, a decrease in the apparent half-life from about 19 h in the fed or diabetic-insulin substituted to about 11 h in the starved or diabetic rat, respectively, was observed. Injection of Bt2cAMP into glucose-fed animals reduced glucokinase synthesis to basal levels within 90 min, without affecting enzyme activity. It is concluded that in metabolic states associated with elevated levels in tissue cAMP glucokinase synthesis is reduced to basal values and, in addition, its rate of degradation is significantly enhanced.