Topinková E, Srám R J
Folia Biol (Praha). 1982;28(5):302-10.
The sensitivity of the peripheral lymphocytes and bone marrow cells to the induction of chromosome aberrations was studied in rats using a model mutagen Cyclophosphamide. In the acute-exposure experiment the rats were treated with CY by the intraperitoneal injection of 10, 20, and 40 mg.kg-1 b.w. A clear-cut dose-dependence was found in the frequency of aberrant cells as well as in the values of breaks per cell in both analysed cell types. In the chronic experimental series CY was administered to animals in drinking water for 28 days as 0.001%, 0.002%, 0.004%, and 0.008% CY. CY increased the frequency of aberrant metaphases and breaks per cell depending on its concentration identically in the peripheral lymphocytes and bone marrow cells. The results of acute and chronic exposures indicate that both somatic tissue cells are equally susceptible to the clastogenic action of CY.
使用模型诱变剂环磷酰胺，在大鼠中研究了外周淋巴细胞和骨髓细胞对染色体畸变诱导的敏感性。在急性暴露实验中，通过腹腔注射10、20和40mg·kg-1体重的环磷酰胺对大鼠进行处理。在两种分析的细胞类型中，异常细胞的频率以及每个细胞的断裂值均呈现明显的剂量依赖性。在慢性实验系列中，以0.001%、0.002%、0.004%和0.008%的环磷酰胺浓度，将其添加到动物饮用水中持续28天。环磷酰胺根据其浓度，使外周淋巴细胞和骨髓细胞中异常中期相的频率以及每个细胞的断裂数增加。急性和慢性暴露的结果表明，两种体细胞组织对环磷酰胺的致断裂作用同样敏感。
