Kodýtková I, Madar J, Srám R J
Folia Biol (Praha). 1980;26(2):94-102.
The frequency of chromosomal aberrations in bone marrow cells and the primary immne response to sheep erythrocytes were studied at weekly intervals in mice exposed to cyclophosphamide (0.01% or 0.02% concentration in drinking water) and/or alpha-tocopherol(1000 mg/kg intraperitoneally, twice a week) throughout 4 weeks. Both 0.01% and 0.02% CY concentrations induced approximately the same levels of aberrant cells (7.5 and 9.2%, respectively). No significant rise in the frequency of aberrant cells was observed during the four-week course of experiment. The lower (0.01%) concentration of CY significantly increased IgM haemagglutinin titres, while IgG titres decreased rapidly after 3 weeks of exposure; 0.02% CY suppressed almost completely both IgM and IgG antibody levels already after one week of treatment. Simultaneous application of alpha-tocopherol significantly increased the frequency of aberrant cells in 0.01% Cy-treated mice but had no effect on 0.02% CY-treated group. No considerable changes in antibody titres were induced by alpha-tocopherol in both CY-treated and untreated animals.
在为期4周的时间里，每周对暴露于环磷酰胺（饮用水中浓度为0.01%或0.02%）和/或α-生育酚（腹腔注射1000mg/kg，每周两次）的小鼠的骨髓细胞染色体畸变频率以及对绵羊红细胞的初次免疫反应进行研究。0.01%和0.02%的环磷酰胺浓度诱导产生的异常细胞水平大致相同（分别为7.5%和9.2%）。在为期四周的实验过程中，未观察到异常细胞频率有显著升高。较低浓度（0.01%）的环磷酰胺显著提高了IgM血凝素滴度，而在暴露3周后IgG滴度迅速下降；0.02%的环磷酰胺在治疗1周后几乎完全抑制了IgM和IgG抗体水平。同时应用α-生育酚显著增加了0.01%环磷酰胺处理组小鼠的异常细胞频率，但对0.02%环磷酰胺处理组没有影响。α-生育酚在环磷酰胺处理组和未处理组动物中均未引起抗体滴度的显著变化。
