利用蛋白A亲和层析法分离人免疫球蛋白A
Isolation of human IgA utilizing protein A affinity chromatography.
作者信息
Balint J, Ikeda Y, Nagai T, Terman D S
出版信息
Immunol Commun. 1982;11(4):283-91. doi: 10.3109/08820138209050728.
Human serum IgA was isolated employing a system of G-200 column chromatography, anion exchange (DE-52) chromatography, and passage over protein A and anti-IgG Sepharose 4B columns. Polyacrylamide gel electrophoresis (PAGE) analysis of the isolated immunoglobulin revealed polypeptides corresponding to alpha and light immunoglobulin chains of IgA which were identified immunochemically as IgA. Ultracentrifugation studies revealed that the isolated immunoglobulin co-migrated with 7S IgG markers. This procedure may serve as an alternative to classical isolation procedures of IgA from human serum.
采用G - 200柱色谱、阴离子交换（DE - 52）色谱以及通过蛋白A和抗IgG琼脂糖4B柱的系统，从人血清中分离出了人血清IgA。对分离出的免疫球蛋白进行聚丙烯酰胺凝胶电泳（PAGE）分析，结果显示出与IgA的α免疫球蛋白链和轻免疫球蛋白链相对应的多肽，这些多肽经免疫化学鉴定为IgA。超速离心研究表明，分离出的免疫球蛋白与7S IgG标志物共同迁移。该方法可作为从人血清中分离IgA的传统方法的替代方法。
Zotero 插件