通过用不同pH值步骤从蛋白A-琼脂糖柱上洗脱IgG所获得的IgM和IgA污染情况的研究。
Studies of the IgM and IgA contamination obtained by eluting IgG from protein A-Sepharose column with pH steps.
作者信息
Vidal M A, Conde F P
出版信息
J Immunol Methods. 1980;35(1-2):169-72. doi: 10.1016/0022-1759(80)90161-1.
PMID:7204988
Abstract
The IgG fraction obtained from pooled human plasma by eluting a protein A column with a buffer at pH 2.5 was contaminated with approximately 30% of the IgM originally present in the sample. Both the IgM and the IgA contamination can be reduced and the IgG recovery maintained at 90% of the bound IgG when elution of the column is performed at pH 4.0
摘要
通过用pH 2.5的缓冲液洗脱蛋白A柱从混合人血浆中获得的IgG级分被样品中最初存在的约30%的IgM污染。当在pH 4.0进行柱洗脱时，IgM和IgA污染均可减少，且IgG回收率可维持在结合IgG的90%。
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