Bulatov A A, Osipova T A
Int J Pept Protein Res. 1982 Oct;20(4):396-9. doi: 10.1111/j.1399-3011.1982.tb00905.x.
Seiwhale somatotropin has been isolated from seiwhale pituitaries. It was cleaved by cyanogen bromide, trypsin and chymotrypsin. The peptide fragments were separated and purified by gel filtration on Sephadexes, ion exchange chromatography, high voltage electrophoresis and paper chromatography. Amino acid sequences of the isolated peptides were studied by the dansyl-Edman procedure. The data obtained suggested a primary structure of seiwhale somatotropin consisting of 190 amino acid residues and showed a high degree of homology with somatotropins of many other species.
鳁鲸生长激素已从鳁鲸垂体中分离出来。它被溴化氰、胰蛋白酶和糜蛋白酶切割。肽片段通过在葡聚糖凝胶上的凝胶过滤、离子交换色谱、高压电泳和纸色谱进行分离和纯化。通过丹磺酰-埃德曼法研究了分离出的肽的氨基酸序列。所获得的数据表明鳁鲸生长激素的一级结构由190个氨基酸残基组成，并且与许多其他物种的生长激素具有高度同源性。
