Elizarova G P, Kolesnichenko L I, Bochkova O P, Pankov Iu A
Biokhimiia. 1982 Apr;47(4):515-27.
The protein hormone prolactin was isolated from sea whale hypophysis and its amino acid sequence was partially characterized. The hormone was hydrolyzed by BrCN and trypsin, the products of hydrolysis were separated and purified by gel filtration on Sephadex G-50, G-100 and G-25, by ion-exchange chromatography on CM-cellulose and on Dowex 50 x 8 and by partition paper chromatography. The structure of the obtained peptides was determined by the Edman method and by carboxypeptidase A hydrolysis. The partial amino acid sequence of sea whale prolactin making up to 75% of the hormone molecule was established.
从海鲸脑垂体中分离出蛋白质激素催乳素，并对其氨基酸序列进行了部分表征。该激素用溴化氰和胰蛋白酶水解，水解产物通过在Sephadex G - 50、G - 100和G - 25上的凝胶过滤、在CM - 纤维素和Dowex 50 x 8上的离子交换色谱以及纸分配色谱进行分离和纯化。通过埃德曼法和羧肽酶A水解确定了所得肽的结构。确定了构成该激素分子75%的海鲸催乳素的部分氨基酸序列。
