Yates J R, Nuss D L
J Biol Chem. 1982 Dec 25;257(24):15030-4.
Incubation of L-929 cells in growth medium containing excess NaCl induces cellular protein synthesis to become resistant to the inhibitory action of growth medium made hypertonic by the addition of Na-acetate, KCl, choline chloride, D-alanine, or sucrose. Hypertonic preincubation also induces resistance to the inhibitory action of the drugs dimethyl sulfoxide, ethanol, and L-1-tosylamido-2-phenylethyl chloromethyl ketone, but not to the action of NaF or the inhibitors of polypeptide chain elongation, emetine and cycloheximide. Induction is independent of the solute used to make the induction medium hypertonic and is not mediated by an elevated intracellular Na+ concentration. In a separate series of experiments, it was determined that the formation of functional methionyl-tRNAf.40 S ribosomal complexes is not impaired by exposure of uninduced cells to hypertonic growth medium. The combined results are evaluated with respect to the mechanism of action of hypertonic growth medium in the inhibition of polypeptide chain initiation and the adaptive mechanisms that animal cells have evolved to deal with alterations in their extracellular environments.
将L-929细胞置于含有过量氯化钠的生长培养基中培养，可诱导细胞蛋白质合成对因添加醋酸钠、氯化钾、氯化胆碱、D-丙氨酸或蔗糖而变为高渗的生长培养基的抑制作用产生抗性。高渗预孵育还可诱导细胞对二甲基亚砜、乙醇和L-1-甲苯磺酰氨基-2-苯乙基氯甲基酮等药物的抑制作用产生抗性，但对氟化钠或多肽链延伸抑制剂依米丁和环己酰亚胺的作用不产生抗性。诱导作用与用于使诱导培养基变为高渗的溶质无关，且不受细胞内钠离子浓度升高的介导。在另一系列实验中，已确定未诱导的细胞暴露于高渗生长培养基中不会损害功能性甲硫氨酰-tRNAf.40 S核糖体复合物的形成。结合这些结果，对高渗生长培养基抑制多肽链起始的作用机制以及动物细胞为应对细胞外环境变化而进化出的适应性机制进行了评估。
