Yates J R, Nuss D L
J Biol Chem. 1982 Apr 10;257(7):3458-61.
As a result of continued incubation of cultured L-929 fibroblasts in growth medium containing excess NaCl, protein synthesis became resistant to the inhibitory effect of hypertonic medium. Protein synthesis became progressively resistant in a linear fashion during the first 6 h of incubation. The level of resistance attained was directly related to the concentration of excess NaCl to which the cells were exposed during the incubation period. Actinomycin D did not prevent the expression of resistance, indicating that de novo synthesis of RNA is not required. Finally, resistance declined to near the preinduction level by 4 h after resistance cells were returned to isotonic medium.
将培养的L-929成纤维细胞在含有过量氯化钠的生长培养基中持续孵育后，蛋白质合成对高渗培养基的抑制作用产生了抗性。在孵育的最初6小时内，蛋白质合成以线性方式逐渐产生抗性。所达到的抗性水平与细胞在孵育期间所接触的过量氯化钠浓度直接相关。放线菌素D并不能阻止抗性的表达，这表明不需要RNA的从头合成。最后，在抗性细胞回到等渗培养基4小时后，抗性下降至接近诱导前水平。
