Sisken J E, Bonner S V, Grasch S D
J Cell Physiol. 1982 Nov;113(2):219-23. doi: 10.1002/jcp.1041130206.
Early studies on the duration of mitotic stages and on metaphase-to-prophase ratios in a number of normal and neoplastic cells indicated that the process of mitosis becomes altered during the course of oncogenesis. However, the nature of these changes and their effects on each of the mitotic stages are still unclear. With the use of time lapse cinemicrography, we have compared the durations of mitotic stages of SV40/WI-38 and SV40/WI-26 cells to those of their normal counterparts and to other nontransformed human fibroblasts. We also examined the relative frequencies of the individual mitotic stages in fixed preparations of WI-38 and SV40/WI-38 cells. The data show that metaphase durations are increased in the transformed cells and as much as 3-4.7-fold in SV40/WI-38 cells compared to WI-38 and other nontransformed cells. Other stages are also prolonged though to lesser degrees. These findings suggest that increased metaphase/prophase ratios observed in many tumors are due to increases in duration of metaphase rather than to shorter prophases, and that increased mitotic indices commonly observed in malignant tumors and sometimes used as an index of growth rate are at least in part due to the prolongation of mitotic stages.
早期对许多正常细胞和肿瘤细胞有丝分裂阶段的持续时间以及中期与前期比例的研究表明，在肿瘤发生过程中，有丝分裂过程会发生改变。然而，这些变化的本质及其对每个有丝分裂阶段的影响仍不清楚。通过使用延时显微电影摄影技术，我们比较了SV40/WI - 38和SV40/WI - 26细胞有丝分裂阶段的持续时间与其正常对应细胞以及其他未转化的人类成纤维细胞的持续时间。我们还检查了WI - 38和SV40/WI - 38细胞固定制剂中各个有丝分裂阶段的相对频率。数据显示，与WI - 38和其他未转化细胞相比，转化细胞的中期持续时间增加，在SV40/WI - 38细胞中增加了3至4.7倍。其他阶段也有延长，不过程度较小。这些发现表明，在许多肿瘤中观察到的中期/前期比例增加是由于中期持续时间增加，而不是前期缩短，并且在恶性肿瘤中常见的、有时用作生长速率指标的有丝分裂指数增加至少部分是由于有丝分裂阶段的延长。
