Electrical callus formation and its osteogenesis.

Three different electric stimulation methods--direct current, electric Teflon film and PMLG piezoelectric film--could induce electrical osteogenesis around Donryu rat femur. The configuration of each type of electrical osteogenesis was different but the histological findings were essentially the same. 3H-thymidine autoradiographic studies were carried out on rat femurs stimulated by direct current to elucidate some mechanisms of electrical osteogensis. The results disclosed that the electric stimulation affected not only proliferation of osteoprogenitor cells from the periosteal cells but also differentiation of osteoprogenitor cells to osteoblasts and finally to osteocytes.