Gray J C, Elves M W
Clin Orthop Relat Res. 1982 Mar(163):261-71.
Osteogenesis in subcutaneous, cancellous bone isografts in the rat was assessed at 16 days from the uptake of 85Sr. by comparing the uptake in grafts devoid of one or more cellular components, the contribution of each to osteogenesis was estimated. The per cent contribution of each of the osteogenetic elements was: endosteal lining cells together with marrow stroma, 60%; periosteal cells, 30%; free, hemopoietic marrow cells and osteocytes, no significant contribution. These findings are compared with those previously reported for cortical bone isografts and it appears that the rate of osteogenesis is 2.2 times higher in cancellous grafts than in cortical grafts of the same weight, but only 1.7 times higher when grafts of the same volume are compared. Osteogenetic and hemopoietic activities associated with marrow appear to arise from different groups of cells. It is shown how, with iliac bone marrow, these two groups of cells may be separated physically, the hemopoietic cells being removed by means of a jet of saline, and the osteogenetic cells by subsequent enzyme treatment plus a second jet of saline. The osteogenetic and hemopoietic elements were difficult to separate from bone marrow within the femoral diaphysis.
在大鼠皮下松质骨同种异体移植中，于摄取85Sr后16天评估成骨情况。通过比较不含一种或多种细胞成分的移植物中的摄取情况，估算每种成分对成骨的贡献。各成骨元素的贡献百分比为：骨内膜衬里细胞与骨髓基质，60%；骨膜细胞，30%；游离的造血骨髓细胞和骨细胞，无显著贡献。将这些发现与先前报道的皮质骨同种异体移植的发现进行比较，结果显示，相同重量的松质骨移植物的成骨速率比皮质骨移植物高2.2倍，但当比较相同体积的移植物时，仅高1.7倍。与骨髓相关的成骨和造血活动似乎源于不同的细胞群。文中展示了如何利用髂骨骨髓将这两组细胞物理分离，造血细胞通过盐水喷射去除，而成骨细胞通过后续酶处理加第二次盐水喷射去除。在股骨干内，成骨和造血成分难以从骨髓中分离。
