Cervenka J, Osmundsen H
J Lipid Res. 1982 Nov;23(8):1243-6.
A novel procedure is described for the synthesis of acylcarnitines. This entails the synthesis of an N-acyl imidazole intermediate, which subsequently is reacted with L-carnitine perchlorate to form the acylcarnitine. The procedure is rapid and simple, and does not require a large excess of N-acyl imidazole to achieve a reaction yield of 50-70%. A procedure, based on reverse-phase Sep-Pak cartridges for rapid isolation of acylcarnitines from these reaction mixtures is also described. These procedures have, in particular, been applied in the synthesis of polyunsaturated acylcarnitines, including docosahexaenoylcarnitine.
描述了一种合成酰基肉碱的新方法。该方法需要合成N-酰基咪唑中间体，随后该中间体与高氯酸L-肉碱反应形成酰基肉碱。该方法快速且简单，不需要大量过量的N-酰基咪唑就能达到50-70%的反应产率。还描述了一种基于反相Sep-Pak柱从这些反应混合物中快速分离酰基肉碱的方法。这些方法尤其已应用于多不饱和酰基肉碱的合成，包括二十二碳六烯酰基肉碱。
