Montgomery J A, Mamer O A
McGill University, Medical Research Council of Canada Biomedical Mass Spectrometry Unit, Montreal, Quebec.
Anal Biochem. 1989 Jan;176(1):85-95. doi: 10.1016/0003-2697(89)90277-7.
A method for determining urinary concentrations of carnitine and acylcarnitine esters is described that employs fast atom bombardment mass spectrometry, stable isotope dilution techniques, and a novel deutero-methyl esterification that permits unambiguous identification and quantitation of free carnitine and acylcarnitines. It is rapid, does not require chromatographic or other isolation procedures, and is immune to analyte losses in sample preparation. Urinary concentrations are reported for adult control subjects and for others with various metabolic disorders.
本文描述了一种测定尿液中肉碱和酰基肉碱酯浓度的方法，该方法采用快原子轰击质谱法、稳定同位素稀释技术以及一种新型的氘代甲基酯化方法，可对游离肉碱和酰基肉碱进行明确的鉴定和定量。该方法快速，无需色谱或其他分离程序，且不受样品制备过程中分析物损失的影响。文中报告了成年对照受试者以及患有各种代谢紊乱疾病的其他人群的尿液浓度。
