Paradinas F J, Southcott B M, O'Connell D, den Hollander F, Schuurs A H, Pulley M S, Dumonde D C
J Pathol. 1982 Dec;138(4):309-23. doi: 10.1002/path.1711380403.
Lymphokines (LCL-LK) prepared from the human lymphoid cell line RPMI 1788 were injected into dermal nodular metastases of three patients with advanced breast carcinoma anergic to recall antigen (tuberculin). Three different injection schedules were employed. Ten such nodules were examined by excision biopsy and their histological appearances were compared with biopsies of four further nodules not injected with the lymphokine. Intranodular injection of LCL-LK resulted in clinical regression of tumour and histological evidence of tumour-cell necrosis with pleomorphic leucocytic infiltration by polymorphs, macrophages and lymphocytes. Electron microscopy of two such nodules failed to show close cell contact between leucocytes and tumour cells prior to tumour-cell necrosis. This study extends evidence that injection of inflammatory lymphokines into accessible tumour can result in local tumour regression and it suggests that such tumour-cell destruction may be the result of a variety of factors operating during local inflammatory response.
从人淋巴母细胞系RPMI 1788制备的淋巴细胞激活因子（LCL-LK）被注射到3例对回忆抗原（结核菌素）无反应的晚期乳腺癌患者的皮肤结节性转移灶中。采用了三种不同的注射方案。通过切除活检检查了10个这样的结节，并将其组织学表现与另外4个未注射淋巴细胞激活因子的结节的活检结果进行了比较。结节内注射LCL-LK导致肿瘤临床消退，并有肿瘤细胞坏死的组织学证据，伴有多形核白细胞、巨噬细胞和淋巴细胞的多形性白细胞浸润。对两个这样的结节进行电子显微镜检查，未发现肿瘤细胞坏死前白细胞与肿瘤细胞之间有紧密的细胞接触。这项研究进一步证明，将炎性淋巴细胞激活因子注射到可触及的肿瘤中可导致局部肿瘤消退，并提示这种肿瘤细胞破坏可能是局部炎症反应过程中多种因素作用的结果。
