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胎儿三毛滴虫的表面电荷

The surface charge of Tritrichomonas foetus.

作者信息

Costa e Silva Filho F, Elias C A, de Souza W

出版信息

J Protozool. 1982 Nov;29(4):551-5. doi: 10.1111/j.1550-7408.1982.tb01333.x.

Abstract

The surface charge of Tritrichomonas foetus was evaluated by means of the binding of colloidal iron hydroxide particles at pH 1.8 and cationized ferritin particles at pH 7.2 to the cell surface, as visualized by electron microscopy and by direct measurements of the electrophoretic mobility (EPM), of cells suspended in solutions of different ionic strength and pH. At pH 7.2, T. foetus has a negative surface charge with a mean EPM of -1.03 micrometer . s-1 . V-1 . cm. At lower pH, there is a decrease in the negative surface charge with an isoelectric point at pH 1.2. At higher pH (greater than 9.0), there is an increase in the surface charge reaching an EPM of -2.5 micrometers . s-1 . V-1 . cm. These results indicate that the surface of T. foetus contains both negatively and positively charged dissociating groups. Binding of colloidal iron hydroxide and cationized ferritin particles throughout the cell surface of the protozoon was observed. Treatment of T. foetus with neuraminidase or trypsin reduced significantly the EPM of the cells. Enzyme-treated cells recovered their normal EPM when incubated for 6 h in fresh culture medium by a process that is inhibited by puromycin.

摘要

通过在pH 1.8下将氢氧化铁胶体颗粒和在pH 7.2下将阳离子化铁蛋白颗粒结合到胎儿三毛滴虫的细胞表面来评估其表面电荷,通过电子显微镜观察以及直接测量悬浮在不同离子强度和pH溶液中的细胞的电泳迁移率(EPM)来进行评估。在pH 7.2时,胎儿三毛滴虫具有负表面电荷,平均EPM为-1.03微米·秒⁻¹·伏⁻¹·厘米。在较低pH下,负表面电荷减少,等电点在pH 1.2。在较高pH(大于9.0)时,表面电荷增加,EPM达到-2.5微米·秒⁻¹·伏⁻¹·厘米。这些结果表明,胎儿三毛滴虫的表面含有带负电荷和正电荷的解离基团。观察到氢氧化铁胶体和阳离子化铁蛋白颗粒在原生动物的整个细胞表面结合。用神经氨酸酶或胰蛋白酶处理胎儿三毛滴虫会显著降低细胞的EPM。酶处理的细胞在新鲜培养基中孵育6小时后通过一种被嘌呤霉素抑制的过程恢复其正常EPM。

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