Costa e Silva Filho F, Elias C A, de Souza W
Cell Biophys. 1986 Jun;8(3):161-76. doi: 10.1007/BF02788492.
The surface charge of three strains of Trichomonas vaginalis and five strains of Tritrichomonas foetus was determined by direct measurement of the mean cellular electrophoretic mobility (EPM) of cells suspended in solutions of different ionic strength and pH. No differences were observed in the mean EPM among the two species, although significant differences among the strains exist. Strains that are more pathogenic to mouse, as measured using the subcutaneous assay, had a surface more negative. Treatment of the parasites with trypsin or neuraminidase reduced significantly their mean EPM and increased their isoelectric point. Tritrichomonas foetus was more sensitive to the enzyme treatment than T. vaginalis. Enzyme-treated cells recovered their normal EPM if, after enzyme treatment, they were incubated in fresh culture medium. The recovery process of trypsin-treated cells was inhibited 10-20% by addition of inhibitors of either protein synthesis (puromycin) or N-glycosylation of proteins (tunicamycin) to the incubation medium, suggesting that a cytoplasmic pool of sialoglycoproteins may exist. The recovering of the EPM of T. foetus and T. vaginalis previously treated with neuraminidase was inhibited by puromycin or tunicamycin about 40-50% and 17-30%, respectively. These observations suggest that sialoglycolipids exist on the surface of both parasite species, and that they contribute more to the surface charge of T. vaginalis than to that of T. foetus.
通过直接测量悬浮于不同离子强度和pH值溶液中的细胞平均细胞电泳迁移率（EPM），测定了三株阴道毛滴虫和五株胎儿三毛滴虫的表面电荷。尽管各菌株之间存在显著差异，但两种滴虫的平均EPM未观察到差异。使用皮下试验测定，对小鼠致病性更强的菌株表面更负。用胰蛋白酶或神经氨酸酶处理寄生虫可显著降低其平均EPM并提高其等电点。胎儿三毛滴虫比阴道毛滴虫对酶处理更敏感。如果酶处理后的细胞在新鲜培养基中孵育，它们会恢复正常的EPM。向孵育培养基中添加蛋白质合成抑制剂（嘌呤霉素）或蛋白质N-糖基化抑制剂（衣霉素），胰蛋白酶处理细胞的恢复过程会受到10%-20%的抑制，这表明可能存在唾液酸糖蛋白的细胞质池。嘌呤霉素或衣霉素分别约40%-50%和17%-30%抑制先前用神经氨酸酶处理的胎儿三毛滴虫和阴道毛滴虫EPM的恢复。这些观察结果表明，两种寄生虫表面均存在唾液酸糖脂，且它们对阴道毛滴虫表面电荷的贡献比对胎儿三毛滴虫的贡献更大。
