Silva Filho F C, De Souza W
J Protozool. 1986 Feb;33(1):6-10. doi: 10.1111/j.1550-7408.1986.tb05546.x.
Drugs that interact with microtubules (colchicine and vinblastine) and microfilaments (cytochalasin B) partially inhibited cell growth and motility of Tritrichomonas foetus. Parasites incubated with these substances became rounded and cell division was blocked. Neither colchicine nor vinblastine disrupted the microtubules that form the peltar-axostylar system. Any one of these drugs interfered with the net negative surface charge of T. foetus as evaluated by determination of the cellular electrophoretic mobility (EPM). The decrease in the EPM of cytochalasin B-treated cells was caused by dimethylsulfoxide, which was used as solvent. Untreated cells as well as cytochalasin B-treated cells showed a uniform distribution of anionic sites on the plasma membrane as seen with cationized ferritin particles. In cells treated with colchicine or vinblastine the anionic sites were distributed in patches. These results are discussed in terms of participation of labile cytoplasmic microtubules and microfilaments in the control of the distribution of anionic site-containing macromolecules located on the cell surface of T. foetus.
与微管（秋水仙碱和长春碱）及微丝（细胞松弛素B）相互作用的药物部分抑制了胎儿三毛滴虫的细胞生长和运动。用这些物质孵育的寄生虫变得圆钝，细胞分裂受阻。秋水仙碱和长春碱均未破坏形成皮膜-轴柱系统的微管。通过测定细胞电泳迁移率（EPM）评估，这些药物中的任何一种都会干扰胎儿三毛滴虫的净负表面电荷。细胞松弛素B处理的细胞EPM降低是由用作溶剂的二甲基亚砜引起的。如用阳离子铁蛋白颗粒所见，未处理的细胞以及细胞松弛素B处理的细胞在质膜上显示阴离子位点均匀分布。在用秋水仙碱或长春碱处理的细胞中，阴离子位点呈斑块状分布。根据不稳定的细胞质微管和微丝参与控制位于胎儿三毛滴虫细胞表面的含阴离子位点大分子的分布来讨论这些结果。
