Waites G T, Bell S C
J Reprod Fertil. 1982 Nov;66(2):563-9. doi: 10.1530/jrf.0.0660563.
Intrauterine instillation of heat-deaggregated glycogen in virgin mice induced a marked but transient luminal infiltration of polymorphonuclear leukocytes (PMNL). Similar injections of glycogen 2 days post coitum resulted in termination of pregnancy in the majority of treated females. Embryos recovered on Day 4 of pregnancy from the glycogen-treated females, before the expected time of implantation, had developed to the cavitated blastocyst stage, and were capable of undergoing characteristic outgrowth when cultured in vitro. A small proportion, however, appeared abnormal and did not develop in vitro. Cavitated blastocysts obtained from control mated females did not undergo outgrowth in vitro in the presence of intact PMNL, homogenates of these cells or a low molecular weight (less than 1500) fraction of the homogenates and after culture appeared similar to the abnormal blastocysts recovered from glycogen-treated uteri. It is proposed that the anti-fertility effect of PMNL infiltrates is due to a low molecular weight component of these cells which acts in utero and is cytotoxic to preimplantation blastocysts before their hatching from the zona pellucida.
在未交配的小鼠子宫内注入经热解聚的糖原，可诱导多形核白细胞（PMNL）显著但短暂地浸润至子宫腔。在交配后2天进行类似的糖原注射，导致大多数接受治疗的雌性小鼠终止妊娠。在预期着床时间之前，从接受糖原治疗的雌性小鼠妊娠第4天回收的胚胎，已发育至有空腔的囊胚阶段，并且在体外培养时能够进行典型的生长。然而，一小部分胚胎看起来异常，在体外无法发育。从对照交配雌性小鼠获得的有空腔的囊胚，在完整的PMNL、这些细胞的匀浆或匀浆的低分子量（小于1500）部分存在的情况下，在体外不会生长，并且培养后的情况类似于从接受糖原治疗的子宫中回收的异常囊胚。有人提出，PMNL浸润的抗生育作用是由于这些细胞的一种低分子量成分，其在子宫内起作用，并且在着床前囊胚从透明带孵化之前对其具有细胞毒性。
