Chan P H, Kerlan R, Fishman R A
J Neurosci Res. 1982;8(1):67-72. doi: 10.1002/jnr.490080110.
The intracellular volume of neoplastic brain cells was investigated with regard to the effects of hypo-osmolality and hyperosmolality utilizing double isotopic labeling with 3-0-methyl-D-glucose or tritiated water to measure the total volume of the pellet and inulin or polyethyleneglycol to measure the extracellular volume of the pellet. The cellular pellets were rapidly separated from the incubation medium by centrifugation after addition of an oil mixture. After 60 minutes incubation in Hanks balanced salt medium, the intracellular volume was 7.50 +/- 0.64, 8.48 +/- 0.19, and 2.97 +/- 0.18 ml H2O per 10(6) packed cells for C-6 glioma cells, N18TG-2 neuroblastoma cells, and NG108-15 neuroblastoma X glioma hybrid cells, respectively. The extracellular trapped space of these cultured cells was about one third of the intracellular volume. The intracellular volume of C-6 glioma cells was increased in hypotonic environment, whereas it was decreased with hyperosmolality. Both intracellular sodium and potassium were increased with increased osmolality of the incubation media. These data indicate iso-osmotic regulation by tumor cells, i.e., there is a good correlation between the intracellular volume, intracellular cations and lactate levels of C-6 glioma cells under various osmotic conditions.
利用3-0-甲基-D-葡萄糖或氚标记水进行双同位素标记以测量沉淀的总体积，并用菊粉或聚乙二醇测量沉淀的细胞外体积，研究了低渗和高渗对肿瘤脑细胞细胞内体积的影响。在加入油混合物后，通过离心将细胞沉淀迅速与孵育培养基分离。在汉克斯平衡盐培养基中孵育60分钟后，每10⁶个压实细胞的C-6胶质瘤细胞、N18TG-2神经母细胞瘤细胞和NG108-15神经母细胞瘤X胶质瘤杂交细胞的细胞内体积分别为7.50±0.64、8.48±0.19和2.97±0.18 ml H₂O。这些培养细胞的细胞外截留空间约为细胞内体积的三分之一。C-6胶质瘤细胞的细胞内体积在低渗环境中增加，而在高渗时减少。随着孵育培养基渗透压的增加，细胞内的钠和钾含量均增加。这些数据表明肿瘤细胞存在等渗调节，即在各种渗透条件下，C-6胶质瘤细胞的细胞内体积、细胞内阳离子和乳酸水平之间存在良好的相关性。
