Maton P N, Selden A C, Chadwick V S
Regul Pept. 1982 Oct;4(5):251-60. doi: 10.1016/0167-0115(82)90118-5.
Defining the role of cholecystokinin (CCK) in gut physiology and disease has proved difficult because of problems with development of radioimmunoassays and because CCK exists in several different molecular forms which have different biological actions. In order to measure small (8 amino acid residues, CCK 8) and large (33 and 39 residues) forms of CCK in plasma we have developed high pressure liquid chromatographic (HPLC) fractionation of plasma prior to radioimmunoassay. Fasting plasma CCK 8 and CCK 33/39 levels were usually undetectable (less than 3 and less than 6 pmol/1, respectively). After a liquid fat meal both CCK 8 and CCK 33/39 levels were significantly elevated at 5 min (11.3 +/- 3.3 and 11.6 +/- 2.6 pmol/1, respectively). Peak CCK 8 levels occurred at 30 min (15.0 +/- 4.4 pmol/1) while peak CCK 33/39 levels occurred at 120 min (16.7 +/- 4.9 pmol/1). Total CCK levels showed a biphasic response to the meal. These CCK 8 and CCK 33/39 responses to oral fat are consistent with a role for these hormones in the regulation of gallbladder emptying and pancreatic secretion.
由于放射免疫测定技术发展存在问题，且胆囊收缩素（CCK）存在多种具有不同生物学作用的分子形式，因此确定CCK在肠道生理和疾病中的作用颇具难度。为了测定血浆中CCK的小分子形式（8个氨基酸残基，CCK 8）和大分子形式（33和39个残基），我们在放射免疫测定之前开发了血浆高压液相色谱（HPLC）分级分离法。空腹血浆中CCK 8和CCK 33/39的水平通常无法检测到（分别低于3和低于6 pmol/1）。给予液体脂肪餐后，CCK 8和CCK 33/39的水平在5分钟时均显著升高（分别为11.3±3.3和11.6±2.6 pmol/1）。CCK 8的峰值水平出现在30分钟时（15.0±4.4 pmol/1），而CCK 33/39的峰值水平出现在120分钟时（16.7±4.9 pmol/1）。总CCK水平对该餐呈现双相反应。这些CCK 8和CCK 33/39对口服脂肪的反应与这些激素在调节胆囊排空和胰腺分泌中的作用一致。
