Jakobsen K, Eik-Nes K B
Toxicol Lett. 1982 Sep;13(1-2):113-8. doi: 10.1016/0378-4274(82)90146-1.
When cells from the human cell line NHIK3025 were cultured for 4 days, with hexavalent chromium (K2Cr2O7) at a concentration of 8 . 10(-7)M, the relative cell number was reduced to 38%. This effect of hexavalent chromium was abolished if ascorbic acid and chromate were added simultaneously to the incubation medium, but not if ascorbic acid was added 24 h prior to chromate addition. Dehydroascorbic acid was not able to reduce the effect of K2Cr2O7 either when added simultaneously or when added 24 h prior to chromate exposure. Therefore, the cells could only be protected against the toxic effect of hexavalent chromium when ascorbic acid was added at the same time as K2Cr2O7.
当人类细胞系NHIK3025的细胞在含有浓度为8×10⁻⁷M的六价铬（K₂Cr₂O₇）的培养基中培养4天时，相对细胞数减少至38%。如果在孵育培养基中同时添加抗坏血酸和铬酸盐，六价铬的这种作用就会被消除，但如果在添加铬酸盐前24小时添加抗坏血酸则不会。无论同时添加还是在铬酸盐暴露前24小时添加，脱氢抗坏血酸都不能降低K₂Cr₂O₇的作用。因此，只有当抗坏血酸与K₂Cr₂O₇同时添加时，细胞才能免受六价铬的毒性作用。
