Rodriguez-Martinez H, Einarsson S
Acta Physiol Scand. 1982 Jul;115(3):325-30. doi: 10.1111/j.1748-1716.1982.tb07085.x.
A new dissection technique for the mechanical separation of the smooth muscle layers of the pig oviductal isthmus is described. The spontaneous contractility of the isolated muscle layers was recorded using transducers designed to reflect the proper force development of the tubal muscle tissue specimens under in vitro conditions. A series of well defined stages of the entire estrual cycle was surveyed, and the isolated tubal muscle contractility was compared with that of the intact isthmus, according to previous work. The circular and longitudinal layers are both active during the estrual cycle, showing definite patterns in each stage explored. The circular layer revealed a changing pattern similar to, but of lower activity than, the intact isthmus, specially during the heat, postovulation and luteal phases. The periods are considered relevant because, during heat, the spermatozoa might be actively transported through the isthmus by the concerted contractions and relaxations of the circular muscles. The same would happen during the luteal phase, specially during the time the ova are descending into the uterus. As important would be the postovulation time, during which the sustained contraction of the circular layer could "lock" the ithmic lumen, thus preventing improper timing of ova descent. The longitudinal layer follows the intensity of the contraction patterns of the circular layers, being specially active during the periovulatory period.
本文描述了一种用于机械分离猪输卵管峡部平滑肌层的新解剖技术。使用专门设计的传感器记录离体肌肉层的自发收缩性，该传感器旨在反映体外条件下输卵管肌肉组织标本的适当力发展。根据先前的研究，对整个发情周期的一系列明确阶段进行了调查，并将离体输卵管肌肉收缩性与完整峡部的收缩性进行了比较。在发情周期中，环形层和纵行层均有活性，在每个研究阶段均呈现出明确的模式。环形层呈现出与完整峡部相似但活性较低的变化模式，特别是在发情期、排卵后和黄体期。这些时期被认为是相关的，因为在发情期，精子可能通过环形肌肉的协同收缩和舒张被主动运输通过峡部。在黄体期也会发生同样的情况，特别是在卵子下降到子宫的时期。排卵后时期同样重要，在此期间环形层的持续收缩可能会“锁定”峡部管腔，从而防止卵子下降时间不当。纵行层遵循环形层收缩模式的强度，在排卵周期前后特别活跃。
