Huijghebaert S M, Eyssen H J
Appl Environ Microbiol. 1982 Nov;44(5):1030-4. doi: 10.1128/aem.44.5.1030-1034.1982.
Clostridium sp. strain S1, an unnamed bile acid-desulfating strain from rat intestinal microflora (S.M. Huijghebaert, J. A. Mertens, and H. J. Eyssen, Appl. Environ. Microbiol. 43:185-192, 1982), was examined for its ability to desulfate different bile acid sulfates and steroid sulfates in growing cultures. Clostridium sp. strain S1 desulfated the 3 alpha-monosulfates of chenodeoxycholic, deoxycholic, and cholic acid, but not their 7 alpha- or 12 alpha-monosulfates. Among the 3-sulfates of the 5 alpha- and 5 beta-bile acids, only bile acid-3-sulfates with an equatorial sulfate group were desulfated. Hence, Clostridium sp. strain S1 desulfated the 3-sulfates of bile acids with a 3 alpha, 5 beta-, a 3 beta, 5 alpha- or a 3 beta, delta 5-structure. In contrast, the bile acid-3-sulfates with a 3 beta, 5 beta- or a 3 alpha, 5 alpha-structure were not desulfated. In addition, Clostridium sp. strain S1 did not hydrolyze the equatorial 3-sulfate esters of C19 and C21 steroids and cholesterol or the phenolic 3-sulfate esters of estrone and estradiol. 23-Nordeoxycholic acid with a C-23 carboxyl group was also not desulfated, in contrast to the 5 beta-bile acid 3 alpha-sulfates with a C-24 or C-26 carboxyl group. Therefore, the specificity of the sulfatase of Clostridium sp. strain S1 is related to the location of the sulfate group on the bile acid molecule, the equatorial orientation of the sulfate group, and the structure of the C-17 side chain, its carboxyl group, and chain length.
梭菌属菌株S1是从大鼠肠道微生物群中分离出的一种未命名的胆汁酸脱硫菌株（S.M. Huijghebaert、J.A. Mertens和H.J. Eyssen，《应用与环境微生物学》43:185 - 192，1982年），对其在生长培养物中使不同胆汁酸硫酸盐和类固醇硫酸盐脱硫的能力进行了检测。梭菌属菌株S1能使鹅去氧胆酸、脱氧胆酸和胆酸的3α - 单硫酸盐脱硫，但不能使其7α - 或12α - 单硫酸盐脱硫。在5α - 和5β - 胆汁酸的3 - 硫酸盐中，只有具有平伏硫酸盐基团的胆汁酸 - 3 - 硫酸盐能被脱硫。因此，梭菌属菌株S1能使具有3α, 5β - 、3β, 5α - 或3β, Δ5 - 结构的胆汁酸的3 - 硫酸盐脱硫。相比之下，具有3β, 5β - 或3α, 5α - 结构的胆汁酸 - 3 - 硫酸盐不能被脱硫。此外，梭菌属菌株S1不会水解C19和C21类固醇及胆固醇的平伏3 - 硫酸酯，也不会水解雌酮和雌二醇的酚性3 - 硫酸酯。与具有C - 24或C - 26羧基的5β - 胆汁酸3α - 硫酸盐不同，具有C - 23羧基的23 - 去氧胆酸也不能被脱硫。因此，梭菌属菌株S1硫酸酯酶的特异性与胆汁酸分子上硫酸基团的位置、硫酸基团的平伏取向以及C - 17侧链的结构、其羧基和链长有关。
