Rizzardini M, Smith A G
Biochem Pharmacol. 1982 Nov 15;31(22):3543-8. doi: 10.1016/0006-2952(82)90573-1.
Male and female F 344 rats were dosed every other day for 103 days with 50 mumole of hexachlorobenzene (HCB)/kg. Females developed a hepatic porphyria, the urine and liver levels of porphyrins being 40- and 310-fold higher respectively than those of males. Urine was periodically hydrolysed and analysed for the three metabolites pentachlorophenol, 2,3,5,6-tetrachlorobenzene-1,4-diol and pentachlorothiophenol (derived from the mercapturate). The combined urinary excretion of these was greater in females than males, especially during the first 10 weeks. Pentachlorothiophenol was particularly high in female urine. After 103 days this metabolite was slightly less in female faeces than in male's but free hepatic pentachlorothiophenol was 3.6-fold greater. Although total 24 hr excretions of metabolites were higher by females than males and after 7 daily doses of HCB, a difference in this respect was not conclusively proven. However, total pentachlorothiophenol excretion was always significantly greater by females. The male/female ratios for pentachlorophenol and pentachlorothiophenol in bile were identical to those for faeces. Excretion of metabolites by both adult males and females was stimulated by pretreatment with diethylstilboestrol (DES). No sex differences in metabolism were observed with immature rats.
将雄性和雌性F344大鼠每隔一天给予50微摩尔/千克的六氯苯（HCB），持续103天。雌性大鼠出现了肝性卟啉症，卟啉的尿液和肝脏水平分别比雄性高40倍和310倍。尿液定期水解，并分析三种代谢物五氯苯酚、2,3,5,6 - 四氯苯 - 1,4 - 二醇和五氯硫酚（来自硫醚氨酸盐）。这些物质的尿液排泄总量在雌性中比雄性中更高，尤其是在最初的10周内。五氯硫酚在雌性尿液中特别高。103天后，这种代谢物在雌性粪便中的含量略低于雄性，但肝脏中游离的五氯硫酚含量是雄性的3.6倍。尽管雌性代谢物的24小时排泄总量高于雄性，并且在每日给予7次HCB后，在这方面的差异尚未得到确凿证实。然而，五氯硫酚的总排泄量在雌性中总是显著更高。胆汁中五氯苯酚和五氯硫酚的雄/雌比率与粪便中的相同。用己烯雌酚（DES）预处理可刺激成年雄性和雌性大鼠代谢物的排泄。未成熟大鼠未观察到代谢方面的性别差异。
