三种细胞分离介质的比较：应用于富集粒系髓细胞系的未成熟细胞
A comparison on 3 cell separation media: application to the enrichment for immature cells of the granular myeloid line.
作者信息
Champelovier P, Seigneurin D
出版信息
Biomed Pharmacother. 1982;36(8-9):378-80.
PMID:7182019
Abstract
Three common cell separation media (Bovine Serum Albumin Percoll and Lymphoprep), used for enrichment for immature cells of the granular myeloid line, were compared for simplicity of use, maximum recovery of Granulo-Macrophagic colony forming cells and myeloblasts, and minimum contamination with other cell types; Percoll, at a density of 1.078 g/cm3, was found to be best suited to our uses.
摘要
比较了三种常用于富集粒系髓样未成熟细胞的常见细胞分离介质（牛血清白蛋白、聚蔗糖-泛影葡胺和淋巴细胞分离液）在使用简便性、粒巨噬细胞集落形成细胞和成髓细胞的最大回收率以及其他细胞类型的最小污染方面的情况；发现密度为1.078 g/cm³的聚蔗糖-泛影葡胺最适合我们的使用。
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