Sirowej H, Krieger R, Kattermann R
J Clin Chem Clin Biochem. 1982 Dec;20(12):875-81.
A method is described for rapid determination of protein-bound carbohydrates in human lipoproteins. The method is based on a successive precipitation of lipoproteins with sodium phosphotungstate and magnesium chloride. After purification of the separated lipoproteins by a twofold reprecipitation, a colorimetric determination of hexoses, hexosamines and N-acetylneuraminic acid (sialic acid) is performed. The coefficients of variation in the series are between 3.2% and 7.3%. The results for lipoprotein-bound carbohydrates after precipitation are compared with the corresponding values after separation by ultracentrifugation. The concentrations of hexoses, hexosamines and N-acetylneuraminic acid in the 3 lipoprotein classes VLDL, LDL and HDL of control persons are reported.
本文描述了一种快速测定人脂蛋白中蛋白质结合碳水化合物的方法。该方法基于用磷钨酸钠和氯化镁连续沉淀脂蛋白。通过两次再沉淀纯化分离出的脂蛋白后，对己糖、己糖胺和N-乙酰神经氨酸（唾液酸）进行比色测定。该系列中的变异系数在3.2%至7.3%之间。将沉淀后脂蛋白结合碳水化合物的结果与超速离心分离后的相应值进行比较。报告了对照组人群极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）这三类脂蛋白中己糖、己糖胺和N-乙酰神经氨酸的浓度。
