Ide P, Billiet G, Bonte J
Bull Assoc Anat (Nancy). 1982 Dec;66(195):465-75.
In 27 postmenopaused patients cellular ER has been assessed after fine needle aspiration by estimation of the cytoplasmic or nuclear fluorescence intensity after incubation with 27 beta-estradiol-6-carboxymethyl oxime-bovine serum albumin-fluorescin isothiocyanate. The cellular changes in the fluorescence intensity and tumor size after a 7 days treatment with Tamoxifen (TMX) have been studied. These factors have been correlated with the anatomoclinical factors. Three different groups of patients can be distinguished; in the first group of 10 patients with predominantly cytoplasmic ER fluorescence before treatment with TMX, tumor reduction and disappearance of the cytoplasmic ER fluorescence are observed in a group with the best anatomoclinical factors. In a second group of 7 patients moderate cytoplasmic and some nuclear ER fluorescence is observed. After the 7 days treatment with TMX tumor reduction is found in cases with diminished nuclear ER fluorescence, on the contrary an increase in nuclear ER fluorescence is found in fast growing tumors, with stationary tumor size after 7 days TMX treatment. A third group of 10 patients displays only nuclear ER fluorescence. After the 7 days treatment with TMX, no change occurred in tumor size, with increase of the nuclear ER fluorescence in 8 patients with fast growing tumors, in 2 patients the diminished nuclear fluorescence was accompanied by a decrease in tumor size.
在27例绝经后患者中，通过与27β-雌二醇-6-羧甲基肟-牛血清白蛋白-异硫氰酸荧光素孵育后，估计细胞质或细胞核荧光强度，对细针穿刺后的细胞雌激素受体（ER）进行了评估。研究了他莫昔芬（TMX）治疗7天后荧光强度和肿瘤大小的细胞变化。这些因素已与解剖临床因素相关联。可区分出三组不同的患者；在第一组10例患者中，TMX治疗前主要为细胞质ER荧光，在解剖临床因素最佳的一组中观察到肿瘤缩小和细胞质ER荧光消失。在第二组7例患者中，观察到中等程度的细胞质和一些细胞核ER荧光。TMX治疗7天后，在细胞核ER荧光减弱的病例中发现肿瘤缩小，相反，在快速生长的肿瘤中发现细胞核ER荧光增加，TMX治疗7天后肿瘤大小稳定。第三组10例患者仅显示细胞核ER荧光。TMX治疗7天后，肿瘤大小无变化，8例快速生长肿瘤患者的细胞核ER荧光增加，2例患者细胞核荧光减弱伴有肿瘤大小减小。
