Kitada S, Hays E F, Mead J F, Zabin I
J Cell Biochem. 1982;20(4):409-16. doi: 10.1002/jcb.240200410.
Extracts of thymic lymphoma that are obtained from AKR mice and are kept in the cold for at least several days can induce lipolytic activity in rat adipocyte suspensions. Freshly prepared extracts have low activity but contain a low molecular weight material of less than 10,000 daltons that aggregates on standing in the cold and becomes active. Treatment of aged extracts with trypsin causes a loss in activity indicating that the active material is a protein. It has been obtained in partially purified form, is relatively heat stable, and is not a lipase. Activity was also demonstrated in AKRXDBA/2 lymphoma (induced by AKR SL3-3 virus) and in transplanted lymphomas from a Friend-virus-induced erythroleukemia cell line in DBA/2 mice, but was not detected in normal thymus, spleen, liver, or other tissues. The partially purified material produced a massive fat mobilization when injected into normal mice.
从AKR小鼠获得并在低温下保存至少数天的胸腺淋巴瘤提取物，可诱导大鼠脂肪细胞悬液中的脂解活性。新鲜制备的提取物活性较低，但含有一种分子量小于10,000道尔顿的低分子量物质，该物质在低温下静置时会聚集并变得活跃。用胰蛋白酶处理老化提取物会导致活性丧失，这表明活性物质是一种蛋白质。它已以部分纯化的形式获得，相对耐热，且不是脂肪酶。在AKRXDBA/2淋巴瘤（由AKR SL3-3病毒诱导）以及DBA/2小鼠中来自Friend病毒诱导的红白血病细胞系的移植淋巴瘤中也证实了活性，但在正常胸腺、脾脏、肝脏或其他组织中未检测到。将部分纯化的物质注射到正常小鼠体内会引起大量的脂肪动员。
