Ireland G W, Stern C D
J Cell Sci. 1982 Dec;58:165-83. doi: 10.1242/jcs.58.1.165.
Cell-substrate contacts in explants of different regions of early chick tissues were investigated using the technique of interference reflection microscopy. All the explants spread as epithelial sheets. During initial spreading a peripheral zone of 2-3 cells formed broad contacts with the substrate. In spread explants some cells in the centre made broad substrate contacts. A mat of extracellular material containing fibronectin was found under the explants. Focal contacts and focal adhesions increased in number during culture, and stress fibres were associated with them. These changes in cell contacts appeared more quickly in some tissues than in others. After 24 h, explants of hypoblast and definitive endoblast could easily be distinguished but by 7 days they were very similar. In the absence of serum, specialized cell contacts developed more quickly; in higher concentrations of serum, more slowly. Confrontations between explants were also examined. The most conspicuous feature was that cells in invading explants normally underlapped invaded cells. Invasion from above by an unspread explant could occur even if the invaded explant had formed many focal adhesions.
利用干涉反射显微镜技术，对早期鸡组织不同区域外植体中的细胞-底物接触进行了研究。所有外植体均铺展成上皮片层。在初始铺展过程中，由2-3个细胞组成的外周区域与底物形成广泛接触。在铺展的外植体中，一些位于中央的细胞也与底物形成广泛接触。在外植体下方发现了含有纤连蛋白的细胞外物质垫。在培养过程中，粘着斑和粘着连接的数量增加，应力纤维与它们相关联。细胞接触的这些变化在某些组织中比在其他组织中出现得更快。24小时后，下胚层和确定内胚层的外植体很容易区分，但到7天时它们非常相似。在无血清的情况下，特化的细胞接触发展得更快；在血清浓度较高时，发展得更慢。还对外植体之间的相互作用进行了检查。最显著的特征是，侵入外植体中的细胞通常位于被侵入细胞之下。即使被侵入的外植体已经形成了许多粘着斑，未铺展的外植体也可能从上方进行侵入。
