Couchman J R, Rees D A
J Cell Sci. 1979 Oct;39:149-65. doi: 10.1242/jcs.39.1.149.
Fibroblasts migrating from heart explants of chick embryos at first have a high rate of locomotion but lack focal contacts or adhesions and also lack substantial actin-containing bundles. A meshwork of 7-nm filaments is present particularly in submembranous regions and is proposed to be directed towards efficient locomotion whilst maintaining a high degree of spreading. Also during the first 48 h there is little production of extracellular fibronectin and the growth rate is low. Later, these fibroblasts develop focal contacts and focal adhesions together with actomyosin bundles, with a parallel increase in fibronectin expression. We propose that progressive immobilization by the development of focal adhesions and actomyosin structures occurs to set these cells up for growth.
从鸡胚心脏外植体迁移出来的成纤维细胞，起初具有较高的运动速率，但缺乏粘着斑或黏附结构，也没有大量含肌动蛋白的束状结构。一种7纳米细丝的网络结构尤其存在于膜下区域，据推测它有助于高效运动，同时保持高度的铺展。同样在最初的48小时内，细胞外纤连蛋白的产生很少，生长速率较低。后来，这些成纤维细胞形成了粘着斑和焦点黏附以及肌动球蛋白束，纤连蛋白的表达也随之平行增加。我们认为，粘着斑和肌动球蛋白结构的形成导致的逐步固定，是为这些细胞的生长做准备。
