Kitamura A
J Cell Sci. 1982 Dec;58:185-99. doi: 10.1242/jcs.58.1.185.
In the ciliate Paramecium caudatum, only mating-reactive cells can attach to the polystyrene surface of Petri dishes. The attachment of mating-reactive cells occurs exclusively at the tips of ventrally located cilia (mating-reactive cilia) and appears to involve a hydrophobic interaction. An increase in the hydrophilic nature of the polystyrene surface following treatment with sulphuric acid results in the loss of attachment of reactive cells. The presence or absence of Ca ions and varying the ionic strength of the medium have no effect upon attachment. Although attachment reactivity is closely correlated with mating reactivity, the attachment sites appear distinct from the mating recognition sites, as suggested by the following experimental results: (I) cells that lose mating reactivity following treatment with trypsin are still able to attach to the polystyrene surface; (2) the attachment of cells appears temperature-dependent over a range where mating reactivity remains uneffected; (3) mating reactivity is quickly lost as a result of attachment to the surface of the dish. While the mechanism of attachment is not the same as sexual recognition, the attachment to the polystyrene surface initiates the early stages of the conjugation process. Cells that attach show micronuclear migration while unattached cells do not. The maximum rate of micronuclear migration occurs approximately 30 min after attachment. Meiosis does not occur following migration in the attached cells as in cells that are sexually mating. The relationship between the mating substance and the attachment substance is discussed, while the utilization of the attachment phenomenon for other experimental procedures is proposed.
在纤毛虫尾草履虫中，只有具有交配反应性的细胞才能附着在培养皿的聚苯乙烯表面。具有交配反应性的细胞的附着仅发生在位于腹侧的纤毛（交配反应性纤毛）的尖端，并且似乎涉及疏水相互作用。用硫酸处理后聚苯乙烯表面亲水性的增加导致反应性细胞附着的丧失。钙离子的存在与否以及培养基离子强度的变化对附着没有影响。虽然附着反应性与交配反应性密切相关，但如以下实验结果所示，附着位点似乎与交配识别位点不同：（1）用胰蛋白酶处理后失去交配反应性的细胞仍能附着在聚苯乙烯表面；（2）在交配反应性不受影响的范围内，细胞的附着似乎与温度有关；（3）由于附着在培养皿表面，交配反应性会迅速丧失。虽然附着机制与性识别不同，但附着在聚苯乙烯表面会启动接合过程的早期阶段。附着的细胞显示出微核迁移，而未附着的细胞则没有。微核迁移的最大速率发生在附着后约30分钟。与进行有性交配的细胞不同，附着细胞迁移后不会发生减数分裂。本文讨论了交配物质与附着物质之间的关系，同时提出了将附着现象用于其他实验程序的方法。
