Nawa T, Ishizeki K, Tachibana T
Arch Histol Jpn. 1978 Apr;41(2):157-65. doi: 10.1679/aohc1950.41.157.
Tooth germ derived from the mandible of newborn mice was used in this study. The enamel organs were dissected and attached directly to coverslips without using the plasma clot. By this method it was possible, for the first time as far as is known, to attain preparations in which the major part of the outgrowth from the explant consisted purely of epithelial-like cells which probably were aneloblasts. Abundant desmosomes and tonofilaments were confirmed by transmission electron microscopy. Under the scanning electron microscope this outgrowth of epithelial cells revealed characteristic intercellular connections which were divided into four types. 1)In the part nearest to the explant, cell boundaries were indicated with a large number of short microvilli. 2)In the zone beyond the first type, the intercellular connection was represented by bridge-like processes firmly combined with each other. 3)In a still further zone, the tips of bridge-like processes were free from the cell connection and extended to the adjacent cells with the advance of cell migration. 4)In the most peripheral part of the explant, the cytoplasmic processes were capable of further expansion. These cells were soon separated from the adjacent cells to migrate as free cells.
本研究使用了源自新生小鼠下颌骨的牙胚。解剖牙釉质器官并直接附着在盖玻片上，不使用血浆凝块。据目前所知，通过这种方法首次有可能获得这样的制剂，即外植体长出物的主要部分纯粹由可能是成釉细胞的上皮样细胞组成。透射电子显微镜证实了大量的桥粒和张力丝。在扫描电子显微镜下，这种上皮细胞的长出物显示出特征性的细胞间连接，可分为四种类型。1）在最靠近外植体的部分，细胞边界有大量短微绒毛。2）在第一类区域之外，细胞间连接由彼此牢固结合的桥状突起表示。3）在更远的区域，随着细胞迁移的推进，桥状突起的尖端与细胞连接分离并延伸到相邻细胞。4）在外植体的最外围部分，细胞质突起能够进一步扩展。这些细胞很快与相邻细胞分离，以游离细胞的形式迁移。
