Dentico P, Baldi L, Trotta F, Buongiorno R, Pastore G, Schiraldi O
Quad Sclavo Diagn. 1982 Sep;18(3):361-7.
Five third generation methods for detection of HBsAg in serum were evaluation: AUSRIA II, Micro-RIA, Hepanosticon, Enzygnost, Antigen-TG. Sera of 340 patients with acute and chronic hepatitis and 160 controls were examined with these techniques. The haemoagglutination and latex tests were less sensitive than the RIA, Micro-RIA and ELISA methods. The RIA procedure remains the more sensitive, as demonstrated with sera diluted. False positive results, frequently depending on the excess of antibodies, were observed in ELISA, haemoagglutination and latex tests. The results confirm that the RIA procedure is still highly specific and sensitive, but ELISA is also a very alternative method for HBsAg detection.
对五种用于检测血清中乙肝表面抗原（HBsAg）的第三代方法进行了评估：AUSRIA II、微量放射免疫测定法（Micro-RIA）、肝酶免疫测定法（Hepanosticon）、酶免疫测定法（Enzygnost）、抗原-TG。采用这些技术对340例急慢性肝炎患者的血清和160例对照者的血清进行了检测。血凝试验和乳胶试验比放射免疫测定法、微量放射免疫测定法和酶联免疫吸附测定（ELISA）法的敏感性低。正如稀释血清所显示的那样，放射免疫测定法仍然是更敏感的方法。在ELISA、血凝试验和乳胶试验中观察到假阳性结果，这些结果常常取决于抗体过量。结果证实，放射免疫测定法仍然具有高度的特异性和敏感性，但ELISA也是一种检测HBsAg的非常好的替代方法。
