Albers-Jackson B, Farris B D, Swindell R T
Curr Eye Res. 1982;2(9):607-11. doi: 10.3109/02713688208996361.
Rabbit lenses were incubated in organ culture with 14C acetate for 3, 6, 12 and 20 hours and the nonsaponifiable fraction, composed primarily of sterols, was isolated. The incorporation of acetate into the nonsaponifiable fraction was linear for periods of 3 to 20 hours incubation. The nonsaponifiable fraction was subjected to thin-layer chromatography and 6 bands were observed. On the basis of Rf values, one band was tentatively identified as squalene, another as lanosterol and a third as a cholesterol-desmosterol mixture. The percentage of the radioactivity incorporated into the cholesterol-desmosterol band increased with incubation time. Conversely the percentage of the radioactivity incorporated into the squalene and lanosterol bands decreased with incubation time, indicating a precursor-product relationship.
将兔晶状体在器官培养中与14C醋酸盐一起孵育3、6、12和20小时，然后分离出主要由甾醇组成的非皂化部分。在3至20小时的孵育期间，醋酸盐掺入非皂化部分的过程呈线性。对非皂化部分进行薄层层析，观察到6条带。根据比移值，一条带初步鉴定为角鲨烯，另一条为羊毛甾醇，第三条为胆固醇-去氢胆固醇混合物。掺入胆固醇-去氢胆固醇带的放射性百分比随孵育时间增加。相反，掺入角鲨烯和羊毛甾醇带的放射性百分比随孵育时间减少，表明存在前体-产物关系。
