Daly M M
J Lipid Res. 1971 May;12(3):367-75.
The synthesis of nonsaponifiable compounds from radioactive mevalonate by segments of adult rat aorta was studied in vitro. The labeled products consisted largely of substances with the chromatographic and chemical behavior of squalene, lanosterol, lathosterol, and cholesterol. Even after 3 or 4 hr of incubation, the incorporation of mevalonate into squalene was higher than its incorporation into C(27) sterols; cholesterol contained less than 20% of the radioactivity in the total sterols. Lanosterol was the most highly labeled sterol. The level of radioactivity in lathosterol was comparable to the level in cholesterol. Small amounts of radioactivity were found in other sterols. Material with the same mobility on TLC as 7-dehydrocholesterol had less radioactivity than cholesterol, but more than sterols with the mobility of desmosterol. The results of measurements made after short periods of incubation showed that squalene and lanosterol became labeled before the other nonsaponifiable compounds.
在体外研究了成年大鼠主动脉片段从放射性甲羟戊酸合成不可皂化化合物的过程。标记产物主要由具有角鲨烯、羊毛甾醇、羊毛甾醇和胆固醇色谱及化学行为的物质组成。即使在孵育3或4小时后，甲羟戊酸掺入角鲨烯的量仍高于其掺入C(27)甾醇的量；胆固醇在总甾醇中的放射性含量不到20%。羊毛甾醇是标记程度最高的甾醇。羊毛甾醇中的放射性水平与胆固醇中的水平相当。在其他甾醇中发现了少量放射性。在薄层层析（TLC）上与7-脱氢胆固醇具有相同迁移率的物质，其放射性低于胆固醇，但高于具有去氢胆固醇迁移率的甾醇。短时间孵育后的测量结果表明，角鲨烯和羊毛甾醇在其他不可皂化化合物之前被标记。
