Hess F G, Cox G E, Bailey D E, Parent R A, Becci P J
J Appl Toxicol. 1981 Aug;1(4):202-9. doi: 10.1002/jat.2550010404.
The effect of 1,3-butanediol on reproductive performance as well as its teratogenic, dominant lethal and cytogenetic effects were studied in five generations of Wistar rats. Animals of both sexes were fed either control diet or diet supplemented with 1,3-butanediol at dose levels of 5, 10 or 24% of the diet by weight. Reproduction and lactation parameters were comparative to controls for four of five generations of dams and pups. In contrast, the pregnancy rate of F1A rats decreased during five successive mating cycles; no pups were obtained in the high-dose level group of the fifth series of litters (F2E generation). Excluding this group, the viability of F2 generation pups revealed no significant differences between litters or between control and test groups. No definitive dose-related teratological findings were found in either soft or skeletal tissue examinations of F3B generation rats. However, incomplete ossification of sternebrae occurred frequently in mid- and high-dose fetuses, whereas missing sternebrae were noted especially in high-level fetuses. Both skeletal tissue findings suggest slight delayed fetal growth. For the dominant lethal assay of the F1B generation, the mutagenic index (percentage resorptions per implant sites) revealed no dose-related trend. In the three-generation cytogenetic study, no 1,3-butanediol related chromosomal aberrations were noted.
在五代Wistar大鼠中研究了1,3 - 丁二醇对生殖性能及其致畸、显性致死和细胞遗传学效应的影响。雌雄动物均喂食对照饮食或添加了1,3 - 丁二醇的饮食，添加剂量分别为饮食重量的5%、10%或24%。在五代母鼠和幼崽中，有四代的繁殖和泌乳参数与对照组相当。相比之下，F1A大鼠在连续五个交配周期中的怀孕率下降；在第五窝高剂量组（F2E代）未获得幼崽。排除该组后，F2代幼崽的存活率在不同窝之间以及对照组和试验组之间均未显示出显著差异。在对F3B代大鼠的软组织或骨骼组织检查中均未发现明确的剂量相关致畸学结果。然而，中高剂量胎儿的胸骨节骨化不全经常发生，而胸骨节缺失尤其在高剂量胎儿中被注意到。这两个骨骼组织结果均表明胎儿生长略有延迟。对于F1B代的显性致死试验，诱变指数（每个植入部位的吸收百分比）未显示出剂量相关趋势。在三代细胞遗传学研究中，未发现与1,3 - 丁二醇相关的染色体畸变。
