Khera K S, Arnold D L, Whalen C, Angers G, Scott P M
Toxicol Appl Pharmacol. 1984 Jul;74(3):345-56. doi: 10.1016/0041-008x(84)90288-6.
Weanling male and female mice (F0) were fed daily diets containing 4-deoxynivalenol (DON) at concentrations that resulted in a dose of 0 or 2.0 mg/kg body wt in Experiment I and 0, 0.375, 0.75, or 1.5 mg/kg body wt in Experiment II. The test diets were continuously fed to the F0 parents and their progeny for the entire duration of these two experiments, which were similar in design. After 30 days of dietary feeding, the mice were allowed to mate within experimental groups for a maximum of three 5-day trials. Females found to have mated successfully were allowed to litter normally. The F1a progeny from 10 dams of each control and 1.5-mg DON/kg groups were cross-fostered at birth, whereas all of the remaining F1a progeny were reared by their natural dams. The progeny were examined until 21 days of age and discarded. The F0 mice were rebred. The females bred to produce the F1b litters were killed on Day 19 of gestation and their fetuses were examined for gross, visceral, and skeletal malformations. Reductions were observed in feed and water intakes and body weight of F0 male and female mice, the number of live pups and postnatal survivors, postnatal body weight of F1a progeny, number of live fetuses, and mean fetal weight of F1b fetuses. No adverse effects on fertility of F0 male and female mice and no major malformations in F1b were found. Cross-fostering offspring between control dams and 1.5-mg DON/kg-treated dams revealed that both postnatal survival and body weight were adversely affected by prenatal exposure as well as by a combined pre- and postnatal exposure. Male and female Sprague-Dawley rats were fed diets containing DON so as to deliver daily doses of 0.25, 0.5, or 1.0 mg/kg body wt. After 6 weeks of feeding, the rats were bred within groups and the males were then discarded. The mated females, maintained on their respective diets for the entire period of pregnancy, were killed on the last day of pregnancy and fetuses evaluated for effects on prenatal development. Except for dilation of renal pelvis and urinary bladder, the significance of which remains undetermined, no other adverse effect was observed.
断奶的雄性和雌性小鼠（F0）每日喂食含有脱氧雪腐镰刀菌烯醇（DON）的日粮，在实验I中，DON浓度导致剂量为0或2.0毫克/千克体重，在实验II中，剂量为0、0.375、0.75或1.5毫克/千克体重。在这两个设计相似的实验的整个过程中，将受试日粮持续喂给F0代亲本及其后代。日粮喂养30天后，让小鼠在实验组内交配，最多进行三次为期5天的交配试验。发现成功交配的雌性小鼠正常产仔。来自每个对照组和1.5毫克DON/千克组的10只母鼠的F1a后代在出生时进行交叉寄养，而其余所有F1a后代由其亲生母鼠抚养。对后代进行检查直至21日龄，然后将其丢弃。F0代小鼠再次繁殖。用于生产F1b窝仔的雌性小鼠在妊娠第19天处死，检查其胎儿的大体、内脏和骨骼畸形情况。观察到F0代雄性和雌性小鼠的采食量、饮水量和体重下降，F1a后代的活仔数和产后存活数、产后体重下降，F1b胎儿的活胎数和平均胎儿体重下降。未发现对F0代雄性和雌性小鼠生育力有不良影响，也未发现F1b有重大畸形。对照组母鼠和1.5毫克DON/千克处理组母鼠之间的交叉寄养后代显示，产前暴露以及产前和产后联合暴露均对产后存活和体重产生不利影响。给雄性和雌性Sprague-Dawley大鼠喂食含有DON的日粮，以使每日剂量达到0.25、0.5或1.0毫克/千克体重。喂养6周后，让大鼠在组内交配，然后将雄性大鼠丢弃。交配后的雌性大鼠在整个妊娠期继续食用各自的日粮，在妊娠最后一天处死，评估胎儿以确定对产前发育的影响。除肾盂和膀胱扩张（其意义尚不确定）外，未观察到其他不良影响。
