Koubek K, Lukásová M
Haematologia (Budap). 1982;15(3):297-301.
A case with a high eosinophilic leukocyte count (100 000/microliter) gradually decreasing to normal in the course of three weeks without any treatment is presented. The highly purified suspension of eosinophilic leukocytes (96 per cent of cells) separated from the peripheral blood following sedimentation with dextran and subsequent centrifugation on 1.148 g/ml density Verografin solution allowed to perform some assays of the presence of membrane antigens. In the complement-mediated cytotoxic reaction using anti T, anti Ia-like and anti AML xenosera we failed to find T, Ia-like antigens and leukaemic antigens on the purified eosinophilic leukocytes.
报告了一例嗜酸性白细胞计数很高(100000/微升)的病例,在未经任何治疗的情况下,其计数在三周内逐渐降至正常。从外周血中分离出高度纯化的嗜酸性白细胞悬液(细胞占96%),该悬液先用右旋糖酐沉淀,随后在密度为1.148 g/ml的Verografin溶液中离心,利用此悬液进行了一些膜抗原存在情况的检测。在使用抗T、抗Ia样和抗急性髓细胞白血病异种血清的补体介导细胞毒性反应中,我们未能在纯化的嗜酸性白细胞上发现T、Ia样抗原和白血病抗原。