Ishii J N, Takagi T, Kameyama K, Nakae T
Tokai J Exp Clin Med. 1982;7 Suppl:157-64.
A method is described for the determination of the molecular weight of the protein moiety of protein surfactant complexes using of low angle laser light (633 nm) scattering in combination with high performance porous silica gel chromatography, precision differential refractometry and differential UV absorption was described. The calibration curves in sodium dodecyl sulfate (SDS) and in a non-ionic surfactant, octaethyleneglycol dodecyl ether, using several reference proteins yielded linear lines. Molecular weight of porin oligomers and monomers, an intrinsic membrance protein that forms the permeability channel in the outer membrane of Escherichia coli, were calculated and were found to be 109,000 and 36,300, respectively in SDS and that of oligomers in octaethy leneglycol dodecyl ether was 114,200. Similarly, the molecular weights of maltoporin oligomers and monomers, which form maltose-maltodextrin specific channels, appeared to be 148,500 and 48,200, respectively, in SDS and that of oligomers in octaethyleneglycol dodeclyl ether was 149,000.
本文描述了一种结合低角度激光（633nm）散射、高效多孔硅胶色谱法、精密示差折射法和紫外吸收差示法来测定蛋白质表面活性剂复合物中蛋白质部分分子量的方法。使用几种参考蛋白绘制的十二烷基硫酸钠（SDS）和非离子表面活性剂八甘醇十二烷基醚中的校准曲线呈线性。计算了孔蛋白寡聚体和单体的分子量，孔蛋白是一种内在膜蛋白，在大肠杆菌外膜中形成通透性通道，在SDS中其寡聚体和单体的分子量分别为109,000和36,300，在八甘醇十二烷基醚中其寡聚体的分子量为114,200。同样，形成麦芽糖-麦芽糊精特异性通道的麦芽糖孔蛋白寡聚体和单体的分子量，在SDS中分别为148,500和48,200，在八甘醇十二烷基醚中其寡聚体的分子量为149,000。
