Dreifuss J J, Gähwiler B
J Physiol (Paris). 1982;78(6):509-13.
The area of the supraoptic nucleus of young rats was cultured for several weeks using the roller-tube technique. Single cell recordings were then obtained with micropipettes filled with the fluorescent dye, lucifer yellow. This allowed intracellular recording of spike activity and the simultaneous ejection of dye for labelling of the cell soma, as well as its dendritic and axonal processes. Some one hundred injected neurones from thirty cultures were examined. A majority of these cells possessed a sizable cell body, 20-45 microns in diameter, from which 1-5 infrequently branching dendrites covered with few or no dendritic spines emerged. This morphology probably characterizes the magnocellular peptidergic neurones of the supraoptic nucleus. Very few of these cells were found to be dye-coupled to neighbouring neurones, but a fair proportion (approximately 10%) apparently gave rise to two axon-like processes.
采用滚管技术对幼鼠视上核区域进行了数周培养。然后用充满荧光染料鲁米诺黄的微吸管进行单细胞记录。这使得能够进行细胞内的锋电位活动记录，并同时注入染料以标记细胞体及其树突和轴突。对来自30个培养物中的约100个注入神经元进行了检查。这些细胞中的大多数具有相当大的细胞体，直径为20 - 45微米，从细胞体发出1 - 5条很少分支的树突，其上很少或没有树突棘。这种形态可能是视上核大细胞肽能神经元的特征。发现这些细胞中很少有与相邻神经元染料偶联的，但相当一部分（约10%）显然产生了两条轴突样突起。
