Gähwiler B H, Dreifuss J J
J Physiol (Paris). 1979 May;75(1):23-6.
Cultures of the hypothalamic supraoptic nucleus area were prepared from 1--10 day old rats by means of the roller-tube technique. After 3 weeks in vitro large nerve cells with long, thin and repeatedly branching axons could be observed. In silver-stained co-cultures of hypothalamic and hypophysial tissue, fibers were seen to bridge the gap between the adjacent explants. Intracellular current injection into the large nerve cells elicited single or repetitive action potentials. Two types of spontaneous discharge patterns were observed. The majority of cells fired randomly and the remainder displayed a phasic dicharge pattern. Simultaneous recordings from 2 cells disclosed that pairs of phasic cells had a tendency to fire in synchronous bursts.
采用滚管技术从1至10日龄大鼠制备下丘脑视上核区培养物。体外培养3周后，可观察到具有长而细且反复分支轴突的大神经细胞。在下丘脑和垂体组织的银染共培养物中，可见纤维连接相邻外植体之间的间隙。向大神经细胞内注入电流可引发单个或重复动作电位。观察到两种自发放电模式。大多数细胞随机放电，其余细胞呈现相位放电模式。对两个细胞的同步记录显示，成对的相位细胞有同步爆发放电的倾向。
