色胺紧张素与血管紧张素的化学特性
Chemical identity of tryptensin with angiotensin.
作者信息
Arakawa K, Yuki M, Ikeda M
出版信息
Biochem J. 1980 Jun 1;187(3):647-53. doi: 10.1042/bj1870647.
Tryptensin, a vasopressor substance generated from human plasma protein fraction IV-4 by trypsin, has been isolated and the amino acid composition analysed. The procedures used for the isolation were: (a) adsorption of the formed tryptensin on Dowex 50W (X2; NH4+ form); (b) gel filtration through Sephadex G-25; (c) cation-exchange chromatography on CM-cellulose; (d) anion-exchange chromatography on DEAE-cellulose; (e) re-chromatography on CM-cellulose; (f) gel filtration on Bio-Gel P-2; (g) partition chromatography on high-pressure liquid chromatography. The homogeneity of the isolated tryptensin was confirmed by thin-layer chromatography and thin-layer electrophoresis. The amino acid analysis of the hydrolysate suggested the following proportional composition: Asp, 1; Val, 1; Ile, 1; Tyr, 1; Phe, 1; His, 1; Arg, 1; Pro, 1. This composition is identical with that of human angiotensin.
胰蛋白酶从人血浆蛋白组分IV - 4生成的血管升压物质——胰蛋白酶升压素已被分离出来，并对其氨基酸组成进行了分析。分离所采用的步骤如下：(a) 将生成的胰蛋白酶升压素吸附在Dowex 50W（X2；NH4+ 型）上；(b) 通过Sephadex G - 25进行凝胶过滤；(c) 在CM - 纤维素上进行阳离子交换色谱；(d) 在DEAE - 纤维素上进行阴离子交换色谱；(e) 在CM - 纤维素上再次色谱；(f) 在Bio - Gel P - 2上进行凝胶过滤；(g) 在高压液相色谱上进行分配色谱。通过薄层色谱和薄层电泳证实了分离出的胰蛋白酶升压素的均一性。水解产物的氨基酸分析表明其比例组成如下：天冬氨酸，1；缬氨酸，1；异亮氨酸，1；酪氨酸，1；苯丙氨酸，1；组氨酸，1；精氨酸，1；脯氨酸，1。该组成与人血管紧张素的组成相同。
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