牛蛙（北美牛蛙）中血管紧张素的化学结构。

Chemical structure of angiotensin in the bullfrog Rana catesbeiana.

作者信息

Hasegawa Y, Watanabe T X, Sokabe H, Nakajima T

出版信息

Gen Comp Endocrinol. 1983 Apr;50(1):75-80. doi: 10.1016/0016-6480(83)90243-5.

PMID:6602082

Abstract

Crude bullfrog angiotensin was prepared by modified Boucher's procedures. The kidney extract was incubated in vitro with homologous plasma. Crude angiotension, 15 micrograms equivalent to [Asp1, Ile5]angiotensin II in rat vasopressor assay, was purified further by three steps by SEP-PAK C18 cartridge, SP-Sephadex column chromatography, and high-performance liquid chromatography on Finepak SIL C18 column. Amino acid analysis was done on 64 ng of the purified material after acid hydrolysis. The fluorescent peptide mapping technique was used to confirm the amino acid sequence. It is proposed that bullfrog angiotensin is [Asp1, Val5, Asn9]angiotensin I.

摘要

粗制牛蛙血管紧张素采用改良的布歇氏方法制备。将肾提取物与同源血浆在体外孵育。在大鼠血管升压试验中，相当于15微克[天冬氨酸1，异亮氨酸5]血管紧张素II的粗制血管紧张素，通过SEP-PAK C18柱、SP-葡聚糖凝胶柱色谱和Finepak SIL C18柱上的高效液相色谱进一步纯化三步。酸水解后，对64纳克纯化物质进行氨基酸分析。采用荧光肽图谱技术确认氨基酸序列。提出牛蛙血管紧张素为[天冬氨酸1，缬氨酸5，天冬酰胺9]血管紧张素I。