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在活体显微镜检查中使用伸缩成像:传统显微镜的一种简单解决方案。

Use of telescopic imaging in intravital microscopy: a simple solution for conventional microscopes.

作者信息

Slaaf D W, Alewijnse R, Wayland H

出版信息

Int J Microcirc Clin Exp. 1982;1(2):121-34.

PMID:7188503
Abstract

Conventional microscopes can be adapted for telescopic imaging by using a projection eyepiece which focuses the image formed by a standard microscope objective at such a large distance that light emanating from points on the object leaves the eyepiece as parallel bundles. A telescopic transfer lens forms the final image in its focal plane independent of the distance between the eyepiece and the transfer lens. In the system reported in this paper, the distance between the eyepiece and transfer lens can be varied from 0 to 280 mm without appreciable vignetting or image degradation for imaging on 35 mm film or smaller formats. This allows great flexibility in the vertical location of the plane of observation while permitting rigid fixation of the recording system-transfer lens combination. By use of a 45 degrees rotatable mirror between the transfer lens and the recording systems, two or more recording systems can be securely mounted and adjusted in advance and rapid selection among them can be made during the course of an experiment.

摘要

传统显微镜可以通过使用投影目镜来适配望远成像,该投影目镜将标准显微镜物镜所形成的图像聚焦在足够远的距离,使得从物体上各点发出的光线以平行光束的形式离开目镜。一个望远转接透镜在其焦平面上形成最终图像,而与目镜和转接透镜之间的距离无关。在本文报道的系统中,目镜和转接透镜之间的距离可以在0至280毫米之间变化,在35毫米胶片或更小尺寸的胶片上成像时,不会出现明显的渐晕或图像质量下降。这在允许记录系统 - 转接透镜组合进行刚性固定的同时,为观察平面的垂直位置提供了极大的灵活性。通过在转接透镜和记录系统之间使用一个45度可旋转镜,可以预先安全地安装和调整两个或更多记录系统,并且在实验过程中可以在它们之间进行快速选择。

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Use of telescopic imaging in intravital microscopy: a simple solution for conventional microscopes.在活体显微镜检查中使用伸缩成像:传统显微镜的一种简单解决方案。
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