细极链格孢和杂色曲霉β-1,3-葡聚糖酶的细胞外系统
The extracellular system of beta-1,3-glucanases of Alternaria tenuissima and Aspergillus vesicolor.
作者信息
Jirků V, Kraxnerová B, Krumphanzl V
出版信息
Folia Microbiol (Praha). 1980;25(1):24-31. doi: 10.1007/BF02876394.
PMID:7188760
Abstract
During cultivation in a minimal medium with glucose Alternaria tenuissima and Aspergillus vesicolor produce constitutively alpha- and beta-glucanases. Fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. Two neutral beta-1,3-glucanases with affinity for yeast glucan were isolated by affinity chromatography and further characterized.
摘要
在以葡萄糖为唯一碳源的基本培养基中培养时，细极链格孢和杂色曲霉可组成型产生α-和β-葡聚糖酶。通过凝胶过滤色谱法分离出对海带多糖具有亲和力的β-1,3-葡聚糖酶组分。通过亲和色谱法分离出两种对酵母葡聚糖具有亲和力的中性β-1,3-葡聚糖酶，并对其进行了进一步表征。
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