黑曲霉的β-葡聚糖水解酶。一种β-(1-4)-葡聚糖水解酶的分离及β-(1-3)-葡聚糖水解酶组分的一些性质
beta-glucan hydrolases from Aspergillus niger. Isolation of a beta-(1-4)-glucan hydrolase and some properties of the beta-(1-3)-glucan-hydrolase components.
作者信息
Clarke A E, Stone B A
出版信息
Biochem J. 1965 Sep;96(3):793-801. doi: 10.1042/bj0960793.
Abstract
- The components of an enzyme preparation from Aspergillus niger, which hydrolysed substrates containing beta-(1-->3)- and beta-(1-->4)-glucosidic linkages, were separated by calcium phosphate and Dowex 1 column chromatography. 2. The hydrolytic activity of each fraction from both types of column towards laminaribiose, laminarin, carboxymethylpachyman, pachydextrins, salicin, cellobiose, cellopentaose and swollen cellulose was tested. 3. The activity towards the beta-(1-->3)-glucosidic substrates was found in three well-separated groups of fractions. The differences in action pattern of these groups is discussed. 4. Preparative-scale chromatography that enabled the separation of a beta-(1-->4)-glucan-glucanohydrolase component substantially free of activity towards beta-(1-->3)-glucosidic substrates is described. Residual beta-(1-->3)-glucan-hydrolase activity was removed by adsorption on to insoluble laminarin at pH3.5.
摘要
- 黑曲霉中一种能水解含有β-(1→3)-和β-(1→4)-糖苷键底物的酶制剂的各组分，通过磷酸钙和Dowex 1柱色谱法进行分离。2. 对来自两种类型柱的各馏分对层二糖、海带多糖、羧甲基茯苓多糖、茯苓糊精、水杨苷、纤维二糖、纤维五糖和膨胀纤维素的水解活性进行了测试。3. 在三组分离良好的馏分组中发现了对β-(1→3)-糖苷底物的活性。讨论了这些组作用模式的差异。4. 描述了一种制备规模的色谱法，该方法能够分离出基本上对β-(1→3)-糖苷底物无活性的β-(1→4)-葡聚糖-葡聚糖水解酶组分。通过在pH3.5下吸附到不溶性海带多糖上，去除了残留的β-(1→3)-葡聚糖水解酶活性。
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